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IGF-I (Insulin-like growth factor 1), Human
Catalog Number: EZC-IG1-H1
Synonym: IGF1, IGF-1, IGF-I, IGF-?, IGF?, IGF1A, IGF-1A, IGFI, Insulin-like growth factor 1, somatomedin C, mechano growth factor, MGF
Source: Recombinant human Insulin-like growth factor 1 (rhIGF-I), Gly49-Ala118 (Accession # P05019) was expressed in human HEK293 cells.
Molecular Characterization: rhIGF-I was fused two amino acid gly-pro at the N-terminal and has a calculated MW of 7.5 kDa. DTT-reduced protein migrates as 8 kDa polypeptide in SDS-PAGE.
Purity: >95% purity as determined by SDS-PAGE of reduced rhIGF-I.
Endotoxin: Less than 1.0 EU endotoxin per g rhIGF-I by the LAL method.
Formulation: Supplied as a 0.22 m filtered solution in 50mM Tris, 100 mM NaCl, pH 8.0 and delivered as liquid formulation or lyophilized powder. Normally mannitol or trehalose are added as protectants before lyophilization. Reconstitution instruction or specific concentrations are included in the hardcopy of CoA along with the products.
Storage: No activity loss was observed after storage at 4-8? for 1 month or -20? for 1 year in lyophilized state. rhIGF-I should be aliquoted and stored at -80? in liquid formulation, for long term storage. Avoid repeated freeze-thaw cycles.
Background: Insulin-like growth factor 1 (IGF-1) also known as somatomedin C, IGF1A, IGFI, sulfation factor [1], and is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin is used for the treatment of growth failure.[2] IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7649 daltons. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. IGF-1 binds to at least two cell surface receptors: the Insulin-like growth factor 1 receptor, abbreviated as "IGF1R", and the insulin receptor. The IGF-1 receptor seems to be the "physiologic" receptor - it binds IGF-1 at significantly higher affinity than the IGF-1 that is bound to the insulin receptor. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. Its primary action is mediated by binding to its specific receptor IGF1R, present on many cell types in many tissues. Binding to the IGF1R, a receptor tyrosine kinase, initiates intracellular signaling; IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and proliferation, and a potent inhibitor of programmed cell death. Insulin-like growth factor 1 has been shown to bind and interact with all the IGF-1 Binding Proteins (IGFBPs), of which there are six (IGFBP1-6). Specific references are provided for interactions with IGFBP3,[3-5] IGFBP4,[6]and IGFBP7.[7]
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
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  Size Price Number
  20 g $364.00
  200 g $1,372.00
Reference
(1) Salmon W, Daughaday W, 1957, J Lab Clin Med 49 (6): 82536. (2) Keating GM, et al., 2008, BioDrugs 22 (3): 17788. (3) Horton JK, et al., 1989, Biochem. Pharmacol. 38 (11): 172736. (4) Ueki I, et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97 (12): 686873. (5) Buckway CK, et al., 2001, J. Clin. Endocrinol. Metab. 86 (10): 494350. (6) Qin X, et al., 1998, J. Biol. Chem. 273 (36): 2350916. (7) Ahmed S, et al, 2003, Biochem. Biophys. Res. Commun. 310 (2): 6128.
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