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Recombinant Proteins
Growth Factors and Cytokines
Receptors
CD Antigens
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G-CSF (Granulocyte colony-stimulating Factor), Human
Catalog Number: EZC-GCF-H1
Synonym: G-CSF, CSF3, CSF-3, C17orf33, CSF3OS, GCSF, MGC45931, Granulocyte colony-stimulating factor
Source: Recombinant human G-CSF / GCSFb (rhG-CSF) Thr2- Pro200, (Accession # NP_757373.1 ) was produced in E.coli cells.
Molecular Characterization: rhG-CSF is fused with an additional Met at the N-terminus has a calculated MW of 18.8 kDa expressed. Protein migrates as 15-20 kDa in reduced SDS-PAGE.
Purity: >98% as determined by SDS-PAGE. All lots are greater than 98% pure.
Endotoxin: Less than 1.0 EU per 1 g of the rhG-CSF by the LAL method.
Formulation: Bulk protein in a 0.22 m filtered solution of 20mM Tris, 20mM NaCl , pH 8.0 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
Storage: Store at -20? in lyophilized state after receipt. For long term storage, upon reconstitution rhG-CSF should be aliquot and store at -20? or -80?. Avoid repeated freeze-thaw cycles.
Background: Granulocyte colony-stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3, CSF3, C17orf33, CSF3OS, GCSF, MGC45931. It is a glycoprotein, growth factor and cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. G-CSF then stimulates the bone marrow to release them into the blood. G-CSF also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF regulates them using Janus kinase (JAK)/signal transducer and activator of transcription (STAT) and Ras /mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signal transduction pathway. G-CSF is produced by endothelium, macrophages, and a number of other immune cells. The natural human glycoprotein exists in two forms, a 174- and 180-amino-acid-long protein of molecular weight 19,600 grams per mole. G-CSF can effect on the hematopoietic system and neuronal cells as a neurotrophic factor. The action of G-CSF in the central nervous system is to induce neurogenesis, to increase the neuroplasticity and to counteract apoptosis.[1, 2] G-CSF stimulates the production of white blood cells (WBC). In oncology and hematology, a recombinant form of G-CSF is used with certain cancer patients to accelerate recovery from neutropenia after chemotherapy, allowing higher-intensity treatment regimens. Another form of recombinant human G-CSF called lenograstim is synthesised in Chinese Hamster Ovary cells (CHO cells). The recombinant human G-CSF synthesised in an E. coli expression system is called filgrastim.
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  20 g $364.00
  200 g $1,680.00
Reference
(1) Schneider A,, et al., 2005, J. Clin. Invest. 115 (8): 208398 (2) Kotenko S.V., et al., 2003, Nat. Immunol. 4:69-77
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