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Recombinant Proteins
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FOLR2 (Folate Receptor beta), Human
Catalog Number: EZC-FO2-H1
Synonym: FOLR2, FOLR-2, BETA-HFR, FBP/PL-1, FR-BETA, FR-P3, Folate-receptor-beta
Source: Recombinant human FOLR2/FBP (rhFOLR2) Thr17-His228 (Accession # P14207-1) fused with a C-terminal his tag was produced in human HEK293 cells.
Molecular Characterization: rhFOLR2 was fused with 6his tag at the C-terminal and has a calculated MW of 26 kDa expressed. The predicted N-terminal is Thr17. Protein migrates as 30-33 kDa in reduced SDS-PAGE resulting from glycosylation.
Purity: >92% as determined by SDS-PAGE. All lots are greater than 90% pure.
Endotoxin: Less than 1.0 EU per 1 g of the rhFOLR2 by the LAL method.
Formulation: Bulk protein in a 0.22 m filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
Storage: Store at -20? in lyophilized state after receipt. For long term storage, upon reconstitution rhFOLR2 should be aliquot and store at -20? or -80?. Avoid repeated freeze-thaw cycles.
Background: Folate receptor beta, also known as Folate receptor 2, FBP, FOLR2, BETA-HFR, FBP/PL-1, FR-BETA, FR-P3, and is a member of the folate receptor (FOLR) family. and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. The FOLR2 protein was originally thought to exist only in placenta, but is also detected in spleen, bone marrow, and thymus. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells. [1,3] It is upregulated on myeloid leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers.[2,4] It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma. [5,6] FOLR2 is a marker for macrophages generated in the presence of M-CSF, but not GM-CSF. Its expression correlates with increased folate uptake ability. Folate conjugates of therapeutic drugs are a potential immunotherapy tool to target tumor-associated macrophages.
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  100 g $470.00
  1 mg $2,106.00
Reference
(1) Ratnam, M. et al., 1989, Biochemistry, 28(20):8249-54. (2) Ross, J.F. et al., 1999, Cancer, 85:348 (3) Reddy, J.A. et al., 1999, Blood, 93:3940 (4) Ross, J.F. et al.,1994, Cancer 73:2432 (5) van der Heijden, J.W. et al., 2009, Arthritis Rheum. 60:12 (6) Nagai, T. et al., 2009, Cancer Immunol. Immunother. Feb 24 epub
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