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ErbB-3 / HER3 (Receptor tyrosine-protein kinase erbB-3), Human, With C-Fc Tag
Catalog Number: EZC-ER3-H2
Synonym: ERBB3, ErbB-3, HER3, HER-3, LCCS2, MDA-BF-1, MGC88033, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3, Receptor tyrosine-protein kinase erbB-3
Source: Recombinant human ErbB3 / HER3 Fc Chimera (rhErbB3-Fc) Met 1-Thr 643 (Accession # NP_001973.2) fused with the C-terminal fused Fc region of human IgG1 was produced in human HEK293 cells.
Molecular Characterization: rhErbB3-Fc is fused with Fc region of human IgG1 at C-terminal and has a calculated MW of 95.4 kDa after removal of signal peptide. The predicted N-terminal is Ser 20. Protein migrates as 130-140 kDa in reduced SDS-PAGE resulting from glycosylation.
Purity: >95% as determined by SDS-PAGE. All lots are greater than 95% pure.
Endotoxin: Less than 1.0 EU per 1 g of the rhErbB3-Fc by the LAL method.
Formulation: Bulk protein in a 0.22 m filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder.Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
Storage: Store at -20? in lyophilized state after receipt. For long term storage, upon reconstitution rhErbB3-Fc should be aliquot and store at -20? or -80?. Avoid repeated freeze-thaw cycles.
Background: ErbB3,also known as Her3 (human epidermal growth factor receptor 3),is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.(1-6).
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  200 g $520.00
  1 mg $1,840.00
Reference
(1) Singer, E. etal., 2001, J. Biol. Chem. 276 (47): 4426674 (2) Kraus,M.H.etal.,1989,Proc.Natl.Acad.Sci.86:9193-9197 (3) Carraway,K.L.etal.,1994,J.Biol.Chem.269:14303-14306 (4) Wallasch,C.etal.,1995,EMBO.J.14:4267-4275 (5) Alimandi,M.etal.,1995,Oncogene.10:1813-1821 (6) Gamett,D.C.etal.,1995,J.Biol.Chem.270:19022-19027
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