Categories
Recombinant Proteins
Growth Factors and Cytokines
Receptors
CD Antigens
Affinity Ligands
Other Proteins
Transfection Reagents
For Cell Lines
For Primary Cells
CXCR4 / CD184 (C-X-C Chemokine Receptor Type 4), Human, With N-GST Tag
Catalog Number: EZC-CX4-H1
Synonym: CXCR4, CD184, D2S201E, FB22, HM89, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM
Source: Recombinant human CXCR4 (rhCXCR4), Met1-Ser46 (Accession # AAH20968.1) was expressed in E.coli cells.
Molecular Characterization: rhCXCR4, fused with GST tag at N-terminal and has a predicted molecular mass of 32.2 kDa. In DTT-reduced SDS-PAGE, rhCXCR4 protein migrates as 33 kDa poly peptide.
Purity: >95% purity as determined by SDS-PAGE of reduced rhCXCR4.
Endotoxin: Less than 0.01ng endotoxin per g rhCXCR4.
Formulation: Bulk protein in a 0.22 m filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
Storage: Store at -20? in lyophilized state after receipt. For long term storage, upon reconstitution rhCXCR4 should be aliquot and store at -20? or -80?. Avoid repeated freeze-thaw cycles.
Background: C-X-C chemokine receptor type 4 also known as fusin or CD184 (cluster of differentiation 184), CXCR4, CD184, D2S201E, FB22, HM89, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R or WHIM. CXCR-4 is an alpha-chemokine receptor specific for stromal-derived-factor-1 (SDF-1 also called CXCL12), a molecule endowed with potent chemotactic activity for lymphocytes. This receptor is one of several chemokine receptors that HIV isolates can use to infect CD4+ T cells. HIV isolates that use CXCR4 are traditionally known as T-cell tropic isolates. Typically, these viruses are found late in infection. It is unclear as to whether the emergence of CXCR4 using HIV is a consequence or a cause of immunodeficiency.CXCR4 is upregulated during the implantation window in natural and hormone replacement therapy cycles in the endometrium, producing, in presence of a human blastocyst, a surface polarization of the CXCR4 receptors suggesting that this receptor is implicated in the adhesion phase of human implantation. SDF-1 and CXCR4 were believed to be a relatively "monogamous" ligand-receptor pair (other chemokines tend to use several different chemokine receptors in a fairly "promiscuous" manner). Recent evidence demonstrates ubiquitin is also a natural ligand of CXCR4.[1] Chronic exposure to THC increased T lymphocyte CXCR4 expression on both CD4+ and CD8+ T lymphocytes. [2] Drugs that block the CXCR4 receptor appear to be capable of "mobilizing" hematopoietic stem cells into the bloodstream as peripheral blood stem cells.
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  50 g $364.00
  1 mg $2,520.00
Reference
(1) Saini V, Marchese A, Majetschak M, 2010, J. Biol. Chem. 285 (20): 1556676. (2) Lecapitaine N, et al. 2011, Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology.
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