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CPE (Carboxypeptidase E), Mouse
Catalog Number: EZC-CAE-M1
Synonym: Carboxypeptidase E, CPE, carboxypeptidase, enkephalin convertase, CPH
Source: Recombinant Mouse Carboxypeptidase E (rmCPE), Met1-Phe476 (Accession # NP_038522) was produced in human HEK293 cells.
Molecular Characterization: rmCPE, a 455 amino acids protein with 6his tag at C-terminus and has a calculated molecular mass of 52 kDa. The predicted N-terminal is Gln28. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmCPE is approximately 52 kDa.
Purity: >95% as determined by SDS-PAGE. All lots are greater than 95% pure.
Endotoxin: Less than 1.0 EU per 1 g of the rmCPE by the LAL method.
Formulation: Bulk protein in a 0.22 m filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder.Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
Storage: Store at -20? in lyophilized state after receipt. For long term storage, upon reconstitution rmCPE should be aliquot and store at -20? or -80?. Avoid repeated freeze-thaw cycles.
Background: Carboxypeptidase E (CPE), also known as Carboxypeptidase H or enkephalin convertase. Carboxypeptidase E cleaves C-terminal amino acid residues and is involved in neuropeptide processing. It is a peripheral membrane protein. CPE specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Carboxypeptidase E appears to have several functions. The active form of carboxypeptidase E was shown to be in secretory vesicles, where it acts as an exopeptidase to activate neuropeptides, It does that by cleaving off basic C-terminal amino acids, producing the active form of the peptide. Products of carboxypeptidase E include insulin, enkephalin, and most other neuroendocrine peptides.It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting signal for regulated secretory proteins in the trans-Golgi network of the pituitary and in secretory granules; regulated secretory proteins are mostly hormones and neuropeptides. Mice with mutant carboxypeptidase E Cpe, display endocrine disorders like obesity and infertility.(1-3).
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  100 g $350.00
  1 mg $2,688.00
Reference
(1) Manser, E. et al., 1990, Biochem. J. 267: 517-525. (2) Jeffrey, K.D. et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105: 8452-8457 (3) Dhanvantari,S. et al., 2000, J. Biol. Chem. 275: 29887-29893
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