| Catalog Number: |
EZC-CD4-H2 |
| Synonym: |
CD4, CD-4, CD4mut, CD-4mut |
| Source: |
Recombinant human CD4 Fc Chimera (rhCD4-Fc) Lys26-Trp390 (Accession # AAH25782) was produced in human HEK293 cells. |
| Molecular
Characterization: |
rhCD4-Fc, fused with Fc fragment Of human IgG1 at the C-terminal and has a calculated MW of 68 kDa expressed. The predicted N-terminal is Lys26. Protein migrates as 80 kDa in reduced SDS-PAGE due to glycosylation. |
| Purity: |
>95% as determined by SDS-PAGE. |
| Endotoxin: |
Less than 1.0 EU per 1 µg of the rhCD4-Fc by the LAL method. |
| Formulation: |
Supplied as a 0.22 µm filtered solution in 50 mM tris, 100 mM glycine, pH7.0 and delivered as liquid formulation or lyophilized powder. Normally mannitol or trehalose are added as protectants before lyophilization.
Reconstitution instruction or specific concentrations are included in the hardcopy of CoA along with the products. |
| Storage: |
No activity loss was observed after storage at 4-8? for 1 month or -20? for 1 year in lyophilized state. rhCD4-Fc should be aliquoted and stored at -80? in liquid formulation, for long term storage. Avoid repeated freeze-thaw cycles. |
| Background: |
Cluster of Differentiation 4 (CD4), also known as T-cell surface antigen T4/Leu-3 (LEU-3) and CD4mut, is a single-pass type I membrane glycoprotein,and is a member of the immunoglobulin superfamily. CD4 expressed on the surface of T helper cells, monocytes, macrophages, and dendritic cells. It has four immunoglobulin domains (D1 to D4) that are exposed on the extracellular surface of the cell: D1 and D3 resemble immunoglobulin variable (IgV) domains. D2 and D4 resemble immunoglobulin constant (IgC) domains. CD4 is a co-receptor that assists the T cell receptor (TCR) with an antigen-presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR by recruiting an enzyme, known as the tyrosine kinase lck, which is essential for activating many molecules involved in the signaling cascade of an activated T cell. CD4 also interacts directly with MHC class II molecules on the surface of the antigen-presenting cell using its extracellular domain. The extracellular domain adopts an immunoglobulin-like beta-sandwich with seven strands in 2 beta sheets, in a Greek key topology.[1] CD4 has also been shown to interact with SPG21 ,[2] Lck[3-4] and Protein unc-119 homolog.[5] CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients. |
| Research Use Only. Not for use in diagnostic or therapeutic procedures. |
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| Reference |
| (1) Brady RL, et al., 1993, Science 260 (5110): 979–83.
(2) Zeitlmann, L., et al., 2001, J. Biol. Chem. (United States) 276 (12): 9123–32.
(3) Rudd CE, et al., 2010, J. Immunol. 185 (5): 2645–9.
(4) Rudd CE, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (14): 5190–4.
(5) Gorska MM, et al., 2004, J. Exp. Med. 199 (3): 369–79. |
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