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CD3D (T-cell surface glycoprotein CD3 delta chain), Human
Catalog Number: EZC-CD3-H1
Synonym: CD3D, CD3-DELTA, T3D, T-cell surface glycoprotein CD3 delta chain
Source: Recombinant human CD3D (rhCD3D) Phe 22-Ala 105 (Accession # AAH39035 ) fused with a C-terminal 6his tag was produced in human HEK293 cells.
Molecular Characterization: rhCD3D, fused with a C-terminal 6his tag and has a calculated MW of 11 KDa. The predicted N-terminal is Phe 22. DTT-reduced protein migrates as 18-25 kDa polypeptide in SDS-PAGE resulting from different glycosylation.
Purity: >95% as determined by SDS-PAGE.
Endotoxin: Less than 1.0 EU per 1 g of the rhCD3D by the LAL method.
Formulation: Supplied as a 0.22 m filtered solution in PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally mannitol or trehalose are added as protectants before lyophilization. Reconstitution instruction or specific concentrations are included in the hardcopy of CoA along with the products.
Storage: No activity loss was observed after storage at 4-8? for 1 month or -20? for 1 year in lyophilized state. rhCD3D should be aliquoted and stored at -80? in liquid formulation, for long term storage. Avoid repeated freeze-thaw cycles.
Background: CD3D, also known as CD3-DELTA, T3D, and is a single-pass type I membrane protein. CD3D is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.[1-3] Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.[4]
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  100 g $530.00
  1 mg $2,240.00
Reference
(1) Gil J, et al., 2011, J Clin Invest, 121(10):3872-6. (2) Lu X, et al.,2011, Hematology, 16(3):185-9. (3) Kim ST, et al., 2010, J Immunol, 185(5):2951-9. (4) Holst, J. et al., 2008, Nat Immunol, 9 (6): 658-66.
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