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CD14, Human
Catalog Number: EZC-CD4-H1
Synonym: CD14, CD-14
Source: Recombinant human CD14 (rhCD14) Thr20-Cys352 (Accession # AAH10507) was produced in human HEK293 cells.
Molecular Characterization: rhCD14, contains eleven leucine-rich repeats regions and five glycosylation sites, and fused with 6his tag at the C-terminal and has a calculated MW of 36.6 kDa expressed. The predicted N-terminal is Thr20. Due to different glycosylation, DTT-reduced protein migrates as 35 -120 kDa polypeptide with the confirmation of Western Blot analysis. and the non-reduced protein migrates as 45-150 kDa protein.
Purity: >90% as determined by SDS-PAGE.
Biological Activity: Measured by its ability to enhance LPS stimulated IL8 secretion by THP-1 human acute monocytic leukemia cells.
Endotoxin: Less than 1.0 EU per 1 g of the rhCD14 by the LAL method.
Formulation: Supplied as a 0.22 m filtered solution in PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally mannitol or trehalose are added as protectants before lyophilization. Reconstitution instruction or specific concentrations are included in the hardcopy of CoA along with the products.
Storage: No activity loss was observed after storage at 4-8? for 1 month or -20? for 1 year in lyophilized state. rhCD14 should be aliquoted and stored at -80? in liquid formulation, for long term storage. Avoid repeated freeze-thaw cycles.
Background: Cluster of differentiation 14 (CD14), is a cell surface glycoprotein, and is a is a component of the innate immune system. CD14 is a myelomonocytic differentiation antigen preferentially expressed on monocytes, macrophages, and activated granulocytes. CD14 exists in two forms. Either it is anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa).[1] CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS).[2-3] CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). CD14 has been proposed to be involved in various biological processes, including transportation of other lipids, cell-cell interaction during different immune responses, as well as recognition of apoptotic cells. Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns. CD14+ cells are monocytes that can differentiate into a host of different cells. CD14 has been shown to interact with Lipopolysaccharide-binding protein.[4-5].
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  100 g $470.00
  1 mg $3,124.00
Reference
(1) Kirkland TN, Viriyakosol S, et al., 1998, Prog. Clin. Biol. Res. 397: 7987. (2) Kitchens RL., 2000, Chem. Immunol.. Chemical Immunology and Allergy 74: 6182. (3) Tapping RI, Tobias PS., 2000, Chem. Immunol.. Chemical Immunology and Allergy 74: 10821. (4) Thomas, Celestine J., et al., 2002, FEBS Lett. (Netherlands) 531 (2): 1848. (5) Yu B, Wright S D., 1995, J. Inflamm. (UNITED STATES) 45 (2): 11525.
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