Recombinant Proteins
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C5a (Complement Component C5a), Human
Catalog Number: EZC-C5A-H1
Synonym: C5a, Complement Component 5a, Complement 5a
Source: Recombinant human Complement Component C5a (rhC5a) Leu679-Arg751 (Accession # P01031) was produced in E.coli cells.
Molecular Characterization: rhC5a has a calculated MW of 8.3 kDa. DTT-reduced protein migrates as estimated 8.3 kDa polypeptide in SDS-PAGE.
Purity: >95% as determined by SDS-PAGE. All lots are greater than 95% pure.
Biological Activity: rhC5a is active based on enzyme release assay of myeloperoxidase. The ED50 = 1.36 nmolar.
Endotoxin: Less than 1.0 EU per 1 g of the rhC5a by the LAL method.
Formulation: Bulk protein in a 0.22 m filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
Storage: Store at -20? in lyophilized state after receipt. For long term storage, upon reconstitution rhC5a should be aliquot and store at -20? or -80?. Avoid repeated freeze-thaw cycles.
Background: Human Complement Component C5a (C5a) is also known as C5,and is a protein fragment released from complement component C5.,is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. (1) There are several functions in the below: C5a is an anaphylatoxin, causing the release of histamine from mast cells; (2)C5a is effective leukocyte chemoattractants, causing the accumulation of white blood cells, especially neutrophil granulocytes, at sites of complement activation;(3)C5a activates white blood cells by increasing avidity for white blood cell integrins and upregulating the Lipoxygenase pathway for arachidonic acid metabolism; C5a is a powerful inflammatory mediator, and seems to be a key factor in the development of pathology of many inflammatory diseases involving the complement system; (4)C5a modulates balance between activating versus inhibitory IgG Fc receptors on leukocytes, thereby enhancing autoimmune response.(5)
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  50 g $430.00
  1 mg $3,360.00
(1) Gerard, C. and N.P. Gerard, 1994,Annu. Rev. Immunol.12:775. (2) Monk, PN. et al., 2007, British Journal of Pharmacology. 152: 429-48. (3) Reis ES, et al. 2011,Brain Behav Immun. Apr 23. (4) Bergseth G,et al. Ann Thorac Surg. 2011 Feb;91(2):527-33. (5) Manthey HD,et al. Int J Biochem Cell Biol. 2009 Nov;41(11):2114-7.
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