Recombinant Proteins
Growth Factors and Cytokines
CD Antigens
Affinity Ligands
Other Proteins
Transfection Reagents
For Cell Lines
For Primary Cells
BAFF / CD257 (B-cell activating factor), Human
Catalog Number: EZC-BAF-H1
Source: Recombinant human BAFF (rhBAFF) Ala134-Leu285 (Accession # AAH20674.1) was produced in Human HEK293 cells.
Molecular Characterization: rhBAFF contains Gly-Pro at N-terminal and has a calculated MW of 17.2 kDa. DTT-reduced protein migrates as 19 kDa polypeptide in SDS-PAGE.
Purity: >96% as determined by SDS-PAGE. All lots are greater than 96% pure.
Endotoxin: Less than 1.0 EU per 1 g of the human BAFF by the LAL method.
Formulation: Bulk protein in a 0.22 m filtered solution of 50 mM tris, 150mM NaCl, pH 7.0 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
Storage: Store at -20? in lyophilized state after receipt. For long term storage, upon reconstitution rhBAFF should be aliquot and store at -20? or -80?. Avoid repeated freeze-thaw cycles.
Background: B-cell activating factor (BAFF), also known as tumor necrosis factor ligand superfamily member 13B , TNFSF13B, BAFF, B Lymphocyte Stimulator (BLyS) , cluster of differentiation 257 (CD257), DTL, TNF- and APOL-related leukocyte expressed ligand (TALL-1), THANK, TNFSF20, ZTNF4, and is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it. These receptors are expressed mainly on mature B lymphocytes (TACI is also found on a subset of T-cells and BCMA on plasma cells). TACI binds worst since its affinity is higher for a protein similar to BAFF, called a proliferation-inducing ligand (APRIL). BCMA displays an intermediate binding phenotype and will work with either BAFF or APRIL to varying degrees. Signaling through BAFF-R and BCMA stimulates B lymphocytes to undergo proliferation and to counter apoptosis. All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors[1], although BAFF has been known to be active as either a hetero- or homotrimer.[2] BAFF acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells.
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase This Product
  Size Price Number
  50 g $470.00
  500 g $2,340.00
(1) Oren,D.A., et al., 2002, Nat. Struct. Biol. 9 (4): 288292. (2) Daridon C, er al.,2008, Autoimmun Rev 7 (4): 26771
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