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ACVR2B (Activin Receptor Type-2B), Human
Catalog Number: EZC-ACB-H1
Synonym: ACVR2B, ACTRIIB, ACTR?B, ActR-IIB, ACTR-?B, MGC116908, Activin receptor type-2B
Source: Recombinant human ACVR2B (rhACVR2B) Met 1- Thr 137 (Accession # NP_001097) was produced in human HEK293 cells.
Molecular Characterization: rhACVR2B contains C-terminal polyhistidine tag and has a calculated MW of 14.5 kDa. The predicted N-terminal is Ser 19. DTT-reduced protein migrates as 25-32 kDa polypeptide in SDS-PAGE.
Purity: >97% as determined by SDS-PAGE. All lots are greater than 97% pure.
Biological Activity: Measured by its ability to inhibit Activin A induced hemoglobin expression in K562 human chronic myelogenous leukemia cells. Approximately 0.3-1 g/mL of rhACVR2B will inhibit 50% of the biological response due to 3 ng/mL of rhActivin A.
Endotoxin: Less than 1.0 EU per 1 g of the rhACVR2B by the LAL method.
Formulation: Bulk protein in a 0.22 m filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
Storage: Store at -20? in lyophilized state after receipt. For long term storage, upon reconstitution rhACVR2B should be aliquot and store at -20? or -80?. Avoid repeated freeze-thaw cycles.
Background: Activin receptor type-2B (ACVR2B),is also known as ActR-IIB and MGC116908, ACVR2B is an activin type 2 receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. This gene encodes activin A type IIB receptor, which displays a 3- to 4-fold higher affinity for the ligand than activin A type II receptor. Defects in ACVR2B are the cause of visceral heterotaxy autosomal type 4 (HTX4).(1,2)
      Research Use Only. Not for use in diagnostic or therapeutic procedures.
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  Size Price Number
  200 g $470.00
  1 mg $1,720.00
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Reference
(1) Attisano, L. et al., 1996, Mol. Cell. Biol. 16: 1066-1073. (2) Woodruff, T.K. (1998) Biochem. Pharmacology 55:953 .
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