What scientists are saying about Avalanche® Transfection Reagents?
Human lymphocyte high efficiency transfection:
"Thanks again for bringing to our lab an efficient transfection reagent that worked on our difficult to transfect BJAB cell line. Results showed Avalanche® reagent to be 10X more efficient than lipofectamine and DharmaFect. We finally have the conditions to work with this cell line by using the Avalanche® Transfection Reagent"
-------A Fernandez, Ph.D. NIH
"It worked like a charm."
Mouse cementoblast OCCm-30 cells. Control plasmid, YFP-N1 plasmid
Avalanche®: 80-90%; Lipofectsamine 2000: 5-10%,
"There was slight toxicity, but it worked like a charm."
------ N Bhattacharyya, Ph.D. NIH
Higher efficiency than Nucleofection:
LA-N-2 neuroblastoma, Ptracer-SV40 (GFP)
Avalanche®: 35% at optimal ratio (1.5 ul/2.5 ul DNA in a six well plate); Nucleofection: 31%
-------C Kaneski, Ph.D, NIH/NINDS
"Ease of use"
"I must say I like this system (Avalanche®), especially its ease of use and the fact that I do not need to change the media anytime after transfection."
--------E Udho, Ph.D (NIH/NINDS)
Great on primary Neuron transfection:
" I tested Avalanche® on primary cortical neurons and it worked much better than any other transfection reagents I've ever tried."
-------A Caputo, PhD, David Geffen School of Medicine at UCLA
"The readings were so high that most wells had overflow readings"
"I got huge transfection efficiency (on using Avalanche®)!
I am using HEKs for BRET ( like FRET but with Renilla as the donor molecule) assays and I am reading Renilla substrate and GFP excitation using a Biotek plate reader. The readings were so high that most wells had overflow readings. I was doing the same assay with Fugene transfected HEK cells an none of the wells were even close to overflow. This means that not just by looking at the microscope but even by a more precise reading at the plate reader your reagent is much superior. So now I have to scale down the amount of plasmids I transfect in HEKs because reagent is just too good!"
-------E Pinceti, PhD, Loyola University Chicago
Tet---On Inducible NSC34 Cells, shRNA rescue by transfecting RNAi Resistant cDNA cloned into an expression vector.
EGFP control plasmid, Avalanche® efficiency 50---60%, Lipofectamine 2000/Less Than 10%, Lipofectamine 3000/Less Than 10%
-------C Peter, Assistant Professor, Icahn School of Medicine at Mount Sinai
C2C12 cells showed big differences between avalanche® and Lipofectamine 2000
--------C Rhodes, Ph.D. NIH/NIDCR
EZ Biosystems™ encourage and welcome feedbacks from scientists in both academia and industry on how to continue to meet the needs and expectations of the life science community.
The following is a selection of the latest relevant references using Avalanche® Transfection Reagents:
Ajuh ET, Wu Z, Kraus E, Weissbach FH, Bethge T, et al. Novel Human Polyomavirus Noncoding Control Regions Differ in Bidirectional Gene Expression according to Host Cell, Large T-Antigen Expression, and Clinically Occurring Rearrangements. J Virol. 2018 Apr 1;92(7)PubMed PMID: 29343574; PubMed Central PMCID: PMC5972868.
Takagi K, Miki Y, Onodera Y, Ishida T, Watanabe M, et al. ARHGAP15 in Human Breast Carcinoma: A Potent Tumor Suppressor Regulated by Androgens. Int J Mol Sci. 2018 Mar 10;19(3)PubMed PMID: 29534468; PubMed Central PMCID: PMC5877665.
Wang S, Allen N, Vickers TA, Revenko AS, Sun H, et al. Cellular uptake mediated by epidermal growth factor receptor facilitates the intracellular activity of phosphorothioate-modified antisense oligonucleotides. Nucleic Acids Res. 2018 Mar 5;PubMed PMID: 29514240; PubMed Central PMCID: PMC5909429.
Lebeau J, Saunders JM, Moraes VWR, Madhavan A, Madrazo N, et al. The PERK Arm of the Unfolded Protein Response Regulates Mitochondrial Morphology during Acute Endoplasmic Reticulum Stress. Cell Rep. 2018 Mar 13;22(11):2827-2836. PubMed PMID: 29539413; NIHMSID: NIHMS952339; PubMed Central PMCID: PMC5870888.
Bennett Saidu NE, Bretagne M, Mansuet AL, Just PA, Leroy K, et al. Dimethyl fumarate is highly cytotoxic in KRAS mutated cancer cells but spares non-tumorigenic cells. Oncotarget. 2018 Feb 6;9(10):9088-9099. PubMed PMID: 29507676; PubMed Central PMCID: PMC5823659.
Garcia-Areas R, Libreros S, Simoes M, Castro-Silva C, Gazaniga N, et al. Suppression of tumor-derived Semaphorin 7A and genetic ablation of host-derived Semaphorin 7A impairs tumor progression in a murine model of advanced breast carcinoma. Int J Oncol. 2017 Nov;51(5):1395-1404. PubMed PMID: 29048670; PubMed Central PMCID: PMC5642386.
Sonar SA, Shaikh S, Joshi N, Atre AN, Lal G. IFN-γ promotes transendothelial migration of CD4+ T cells across the blood-brain barrier. Immunol Cell Biol. 2017 Oct;95(9):843-853. PubMed PMID: 28682305.
Peckys DB, Stoerger C, Latta L, Wissenbach U, Flockerzi V, et al. The stoichiometry of the TMEM16A ion channel determined in intact plasma membranes of COS-7 cells using liquid-phase electron microscopy. J Struct Biol. 2017 Aug;199(2):102-113. PubMed PMID: 28559167.
Zhang YY, Huang YP, Zhao HX, Zhang T, Chen F, et al. Cementogenesis is inhibited under a mechanical static compressive force via Piezo1. Angle Orthod. 2017 Jul;87(4):618-624. PubMed PMID: 28418701.
Seong RK, Seo SW, Kim JA, Fletcher SJ, Morgan NV, Kumar M, Choi YK, Shin OS. Schlafen 14 (SLFN14) is a novel antiviral factor involved in the control of viral replication. Immunobiology. 2017 Jul 11. pii: S0171-2985(17)30115-8. doi: 10.1016/j.imbio.2017.07.002. [Epub ahead of print] PubMed PMID: 28734654.
Kim JA, Park SK, Seo SW, Lee CH, Shin OS. STING is Involved in Antiviral Immune Response against VZV Infection via the Induction of Type I and III IFN Pathways. J Invest Dermatol. 2017 Jun 21. pii: S0022-202X(17)31660-3. doi: 10.1016/j.jid.2017.03.041. [Epub ahead of print] PubMed PMID: 28647346.
Hofmann L, Wang H, Beck A, Wissenbach U, Flockerzi V. A conserved gating element in TRPV6 channels. Cell Calcium. 2017 May;63:24-28. PubMed PMID: 28029385.
Wallbillich JJ, Josyula S, Saini U, Zingarelli RA, Dorayappan KD, et al. High Glucose-Mediated STAT3 Activation in Endometrial Cancer Is Inhibited by Metformin: Therapeutic Implications for Endometrial Cancer. PLoS One. 2017 Jan 23;12(1):e0170318. PubMed PMID: 28114390.
Saidu NE, Noé G, Cerles O, Cabel L, Kavian-Tessler N, et al. Dimethyl fumarate controls the NRF2/DJ-1 axis in cancer cells: therapeutic applications. Mol Cancer Ther. 2017 Jan 9;PubMed PMID: 28069874.
Abraham BJ, Hnisz D, Weintraub AS, Kwiatkowski N, Li CH, et al. Small genomic insertions form enhancers that misregulate oncogenes. Nat Commun. 2017 Feb 9;8:14385. PubMed PMID: 28181482; PubMed Central PMCID: PMC5309821.
Goullet de Rugy T, Bashkurov M, Datti A, Betous R, Guitton-Sert L, et al. Excess Polθ functions in response to replicative stress in homologous recombination-proficient cancer cells. Biol Open. 2016 Oct 15;5(10):1485-1492. PubMed PMID: 27612511; PubMed Central PMCID: PMC5087683.
Renvoisé B, Malone B, Falgairolle M, Munasinghe J, Stadler J, et al. Reep1 null mice reveal a converging role for hereditary spastic paraplegia proteins in lipid droplet regulation. Hum Mol Genet. 2016 Sep 16;PubMed PMID: 27638887.
Chen S, Swier VJ, Boosani CS, Radwan MM, Agrawal DK. Vitamin D Deficiency Accelerates Coronary Artery Disease Progression in Swine. Arterioscler Thromb Vasc Biol. 2016 Aug;36(8):1651-9. PubMed PMID: 27255724; NIHMSID: NIHMS790453; PubMed Central PMCID: PMC4965317.
Baghel KS, Tewari BN, Shrivastava R, Malik SA, Lone MU, et al. Macrophages promote matrix protrusive and invasive function of breast cancer cells via MIP-1β dependent upregulation of MYO3A gene in breast cancer cells. Oncoimmunology. 2016 Jul 15;5(7):e1196299. PubMed PMID: 27622050; PubMed Central PMCID: PMC5006911.
Liu S, Sahid MN, Takemasa E, Kiyoi T, Kuno M, et al. CRACM3 regulates the stability of non-excitable exocytotic vesicle fusion pores in a Ca(2+)-independent manner via molecular interaction with syntaxin4. Sci Rep. 2016 Jun 15;6:28133. PubMed PMID: 27301714; PubMed Central PMCID: PMC4908399.
Zhang YY, Zhao HX, Chen ZB, Lin JX, Liu Y. Osteoprotegerin Promotes Cementoblastic Activity of Murine Cementoblast Cell Line in vitro. Chin J Dent Res. 2016 Jun;19(2):103-8. PubMed PMID: 27379348.
Lear T, McKelvey AC, Rajbhandari S, Dunn SR, Coon TA, et al. Ubiquitin E3 ligase FIEL1 regulates fibrotic lung injury through SUMO-E3 ligase PIAS4. J Exp Med. 2016 May 30;213(6):1029-46. PubMed PMID: 27162139; PubMed Central PMCID: PMC4886359.
Hnisz D, Weintraub AS, Day DS, Valton AL, Bak RO, et al. Activation of proto-oncogenes by disruption of chromosome neighborhoods. Science. 2016 Mar 25;351(6280):1454-8. PubMed PMID: 26940867; NIHMSID: NIHMS783783; PubMed Central PMCID: PMC4884612.
Lee S, Chang J, Blackstone C. FAM21 directs SNX27-retromer cargoes to the plasma membrane by preventing transport to the Golgi apparatus. Nat Commun. 2016 Mar 9;7:10939. PubMed PMID: 26956659; PubMed Central PMCID: PMC4786876.
Toyotome T, Takahashi H, Kamei K. MEIS3 is repressed in A549 lung epithelial cells by deoxynivalenol and the repression contributes to the deleterious effect. J Toxicol Sci. 2016 Feb;41(1):25-31. PubMed PMID: 26763390.
Batista L, Bourachot B, Mateescu B, Reyal F, Mechta-Grigoriou F. Regulation of miR-200c/141 expression by intergenic DNA-looping and transcriptional read-through. Nat Commun. 2016 Jan 4;7:8959. PubMed PMID: 26725650.
Jadhao SJ, Anderson LJ. Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays. Methods Mol Biol. 2016;1442:41-52. PubMed PMID: 27464686.
Naturalium_R. The role of estrogen receptors in prostate cancer development and their contributions in new treatment opportunities. Doctoral Thesis in the Molecular Medicine Study Program at the Georg-August University Göttingen, Göttingen, 2016